diff --git a/setup.h.in b/setup.h.in
index cff68110b4..27c6432a4b 100644
--- a/setup.h.in
+++ b/setup.h.in
@@ -299,8 +299,6 @@
 
 #define wxUSE_MEDIACTRL     0
 
-#define wxUSE_GSTREAMER    0
-
 #define wxUSE_XRC       0
 
 #define wxUSE_XML       wxUSE_XRC